![CytoSurgeâ„¢ 6-Well Plate](https://static.wixstatic.com/media/fdd897_06e9863bb0fc415aa6837e6f8b0b9427~mv2.png/v1/fill/w_439,h_442,al_c,q_85,usm_0.66_1.00_0.01,enc_avif,quality_auto/6-well%20Black.png)
CytoSurgeâ„¢ PCL Scaffolds
CytoSurge™ PCL 6-well inserts are our proprietary 3D cell culture scaffolds made of synthetic polycaprolactone (PCL) biopolymer. Over the past two decades, PCL has been studied and used extensively for tissue engineering applications. It has been cited over 35,000 times in scientific literature.
PCL is,
-
biocompatible and FDA-accepted for medical applications
-
biodegradable
-
compatible with cell-specific polymer blends
CytoSurgeâ„¢ 6-Well Insert
CytoSurge™ Inserts are designed for convenient handling of scaffolds in and out of 6-well plates for media change and a variety of analyses (e.g., PrestoBlue™, Confocal Imaging, etc).
The 6-well plate insert is designed to be a universal fit for most 6-well cell culture plates on the market.
![CytoSurgeâ„¢ 6-well Plate Insert Features](https://static.wixstatic.com/media/fdd897_55c637bf69914be38443715dd4d27cb2~mv2.png/v1/fill/w_246,h_184,al_c,q_85,usm_0.66_1.00_0.01,enc_avif,quality_auto/insert.png)
![fiber diameter](https://static.wixstatic.com/media/fdd897_6b742ad9c0584adba5e5f5db26783ede~mv2.png/v1/fill/w_59,h_77,al_c,q_85,usm_0.66_1.00_0.01,enc_avif,quality_auto/fiber%20dia.png)
fiber diameter ~3 µm
![fiber spacing in the single array of fiber layers](https://static.wixstatic.com/media/fdd897_517c9fedc4b6482d96aaa5056ec46d06~mv2.png/v1/fill/w_47,h_81,al_c,q_85,usm_0.66_1.00_0.01,enc_avif,quality_auto/fiber%20spacing.png)
fiber spacing
~25 µm
![fiber count in single layer fiber array](https://static.wixstatic.com/media/fdd897_1ff7c4bbeec44ef184134ec72a154afe~mv2.png/v1/fill/w_78,h_78,al_c,q_85,usm_0.66_1.00_0.01,enc_avif,quality_auto/fiber%20count.png)
fibers per layer
# 1520
![Layer count](https://static.wixstatic.com/media/fdd897_229cebbbf34446eab0149851e2267e61~mv2.png/v1/fill/w_101,h_68,al_c,q_85,usm_0.66_1.00_0.01,enc_avif,quality_auto/Layer%20count.png)
layers per scaffold
# 4
![The perfusion channel gap between the nanofiber fiber layers in the 3D fiber scaffold](https://static.wixstatic.com/media/fdd897_bc89951c41e246158df925caff8e23c8~mv2.png/v1/fill/w_123,h_68,al_c,q_85,usm_0.66_1.00_0.01,enc_avif,quality_auto/layer%20spacing.png)
layer gap
~ 200 µm
![Surface Area of the Scaffold](https://static.wixstatic.com/media/fdd897_1ff7c4bbeec44ef184134ec72a154afe~mv2.png/v1/fill/w_78,h_78,al_c,q_85,usm_0.66_1.00_0.01,enc_avif,quality_auto/fiber%20count.png)
surface area
A ~ 16.2 cm^2
​
(cell growth area)
![scaffold thickness](https://static.wixstatic.com/media/fdd897_04c7b212d1d240d983a5a23bbccd410a~mv2.png/v1/fill/w_116,h_29,al_c,q_85,usm_0.66_1.00_0.01,enc_avif,quality_auto/scaffold%20thickness.png)
availalble volume
V ~ 0.3 cm^3
![Thickness of the 3D fiber scaffold growth area](https://static.wixstatic.com/media/fdd897_2ea48640b5fb42eba9e13db7660b25f2~mv2.png/v1/fill/w_137,h_55,al_c,q_85,usm_0.66_1.00_0.01,enc_avif,quality_auto/scaffold%20thickness.png)
scaffold thickness
1 mm
![Polymer solution](https://static.wixstatic.com/media/fdd897_d2dec612f5ee440781e6a5e762bda711~mv2.png/v1/fill/w_62,h_68,al_c,q_85,usm_0.66_1.00_0.01,enc_avif,quality_auto/flask.png)
fiber and spacer material
(polycaprolactone)
Visit the store to place order.
![perfusion channel](https://static.wixstatic.com/media/fdd897_9df924a507384712bd0b17c2b4a259ce~mv2.jpg/v1/crop/x_0,y_160,w_879,h_486/fill/w_163,h_90,al_c,q_80,usm_0.66_1.00_0.01,enc_avif,quality_auto/perfusion%20channel.jpg)
Unique linear perfusion channels for mass transport
![3D scaffold](https://static.wixstatic.com/media/fdd897_e569c003f5c743c2816c6bc28756f958~mv2.png/v1/crop/x_0,y_14,w_328,h_291/fill/w_101,h_90,al_c,q_85,usm_0.66_1.00_0.01,enc_avif,quality_auto/3D%20scaffold.png)
Aligned fiber arrays for controlled porosity
Incubate cells with CytoSurge™ over 30 days with healthy proliferation
CytoSurge™ is compatible with Matrigel® or similar materials
(for advanced applications)
![3T3F_PCL_Z-stack](https://static.wixstatic.com/media/fdd897_4036b686773f4837bb11354e0cc22bcf~mv2.png/v1/crop/x_246,y_116,w_1244,h_795/fill/w_276,h_176,al_c,q_85,usm_0.66_1.00_0.01,enc_avif,quality_auto/3T3F_PCL_Image%203_Z-stack_2021-05-03T18-06-07_006_tif.png)
Confocal Microscopy
CytoSurge™ inserts are designed to screen and optimize the adherent cell proliferation conditions for cell manufacturing at scale
![3D cell growth Z-stack projection](https://static.wixstatic.com/media/fdd897_88ef09e5bb2e41bb83b11295cfdde519~mv2.jpg/v1/fill/w_386,h_174,al_c,q_80,usm_0.66_1.00_0.01,enc_avif,quality_auto/3D%20cell%20growth%20hb.jpg)
Multilayered cell growth
Aligned fiber arrays are separated by spacers to achieve uniform cell diffusion, efficient media, gas, and metabolites exchange, confined and confluent cell growth and easy harvesting at the end.
​
![PrestoBlueâ„¢ analysis](https://static.wixstatic.com/media/fdd897_77e989073a814f4f865f90879dba05a4~mv2.png/v1/fill/w_260,h_177,al_c,q_85,usm_0.66_1.00_0.01,enc_avif,quality_auto/presto.png)
Cell analysis with CytoSurge™
CytoSurge™ insert is a convenient feature to move the cells growing on the surface of fibers between different solutions for non-destructive cell monitoring and analysis.